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KMID : 0381120090310040277
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Genes and Genomics
2009 Volume.31 No. 4 p.277 ~ p.282
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Molecular Characterization and Physico-Chemical Analysis of a New Giant Embryo Mutant Allele (get) in Rice (Oryza sativa L.)
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Park Dong-Soo
Jeon Jong-Seong
Park Soo-Kwon
Lee Bong-Chun
Song Song-Yi
Jun Nam-Soo
Manigbas Norvie L.
Cho Jun-Hyun
Nam Min-Hee
Han Chang-Deok
Choi Kyung-Jin
Kim Doh-Hoon
Woo Young-Min
Koh Hee-Jong
Kang Hang-Won
Yi Gi-Hwan
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Abstract
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The rice embryo is rich in lipid and protein bodies, bioactive chemicals such as dietary fiber, phytic acids, vitamin B and E, and gamma aminobutyric acid (GABA) than the endosperm. In this paper, we report a new giant embryo mutant, get, induced from somaclonal variation derived by anther culture in rice. Sequence analysis of Cytochrome P450 of the get mutant revealed that get is a new allele of the GE gene with a single point mutation with substitution of amino acid, W395 to L395. The weight of the get mutant embryo was 3.7 times higher than normal embryo. Tocopherol and mineral content were also higher than the previously reported giant embryo rice variety, Keunnun. These results indicated that this new giant embryo rice (get) offers a promising source of genetic material in improving nutritional quality of rice especially tocopherol, essential minerals, and GABA.
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KEYWORD
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anther culture, GABA, giant embryo mutant, rice, tocopherol
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